Klaida
  • Įvyko klaida įkeliant kanalų duomenys

Biotechnology and Genetic Engineering (BBK 3011)

Course code

Course group

Volume in ECTS credits

Course hours

BBK 3011

C

6

160

Course type (compulsory or optional)

Compulsory

Course level (study cycle)

Bachelor

Semester the course is delivered

Spring

Study form (face-to-face or distant)

Face-to-face

Course title in Lithuanian

BIOTECHNOLOGIJA IR GENŲ INŽINERIJA

Course title in English

BIOTECHNOLOGY AND GENETIC ENGINEERING

Short course annotation in Lithuanian

Biotechnologijos principai ir pagrindinės sąvokos. Mikroorganizmų biotechnologija. Eukariotinių ląstelių ir audinių auginimas. Bioreaktoriai. Biotechnologiniai produktai. Molekulinės biotechnologijos raida. Genetinės manipuliacijos principai. Genų klonavimas. DNR vektoriai. Genetinės transformacijos metodai. Genetiškai modifikuoti organizmai. Biotechnologiniai produktai medicinoje. Vakcinos. Molekulinės diagnostikos produktai. Biotechnologija pramonėje. Žemės ūkio biotechnologija. Aplinkos biotechnologija. Biotechnologijos produktų komercializacijos pagrindai. Biotechnologijos produktų saugumas. Etiniai biotechnologijų klausimai.

Short course annotation in English

Biotechnology principles and concepts. Microbial biotechnology. Eukaryotic cell and tissue culture. Bioreactors. Biotechnological products. Development of molecular biotechnology. Principles of genetic manipulation. Gene cloning. DNA vectors. Methods of genetic transformation. Genetically modified organisms. Biotechnology in medicine. Vaccines. Molecular diagnostics. Industrial biotechnology. Agricultural biotechnology. Environmental biotechnology. Commercialization of biotechnological products. Safety of biotechnological products. Ethical issues in biotechnology.

 Prerequisites for entering the course

Basics in biology, chemistry, biochemistry and genetics is  required.

Course aim

The aim of the course is to introduce principles and concepts of biotechnology and to provide basic knowledge of genetic engineering technology and its applications.

Content (topics)

 1. Principles and concepts of biotechnology
 2. Microbial and eucaryotic cell and tissue culture. Bioreactors.
 3. Principles of molecular biology and genetic transformation
 4. Biotechnological research applications
 5. Commercialization of biotechnological products
 6. Safety and ethical issues in biotechnology

Practical work (contents):

eucaryotic cell and tissue culture; plasmid DNA preparation; DNA amplification and analysis; genetic transofrmation; protein purification; protein separation and analysis techniques.

Distribution of workload for students (contact and independent work hours)

Lectures – 30 hours, laboratory work – 20 hours,  individual work – 58 hours.

Structure of cumulative score and value of its constituent parts

Final assessment sums the assessments of written final examination (50%), written mid-term examination (20%) and assessment of laboratory works (30%).

Recommended reference materials

No.

 

Publication year

Authors of publication and title

Publishing house

Number of copies in

University library

Self-study rooms

Other libraries 

Basic materials

1.

2000

Walker J.M., Rapley R., Molecular biology and biotechnology.

Cambridge : Royal Society of Chemistry

1

 

 

2.

2004

Smith J.E., Biotechnology, 4th edition

Cambridge University Press

 

 

1

3.

2006

Primrose S.B., Twyman R.M., Principles of Gene Manipulation and Genomics, 7th edition

Blackwell Publishing

 

 

1

Supplementary materials

1.

2003

Glick B.R., Pasternak J.J., Molecular Biotechnology: Principles and Applications of recombinant DNA, 3rd edition

ASM Press

 

2.

2006

Ratledge C., Kristiansen B., Basic Biotechnology

Cambridge University Press

 

3.

2002

Nicholl D.S.T., Introduction to genetic engineering, 2nd edition

Cambridge University Press

 

4.

2004

Christou P., Klee H., Handbook of Plant Biotechnology, Vol. 1-2

John Wiley and Sons, Ltd.

 

5.

2006

Kun L.Y., Microbial Biotechnology: Principles and Applications, 2nd edition

World Scientific

 

6.

2005

Seiler J.P., Good laboratory practice, 2nd edition

Springer

 

7.

2004

Parekh S.R., , The GMO Handbook: Genetically Modified Animals, Microbes, and Plants in Biotechnology

Humana Press Inc.

 

Course programme designed by

Dr. Danas Baniulis, Faculty of Natural Sciences

Additional information