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Molecular Genetics (BIO4014)

Course code

Course group

Volume in ECTS credits

Course hours

BIO 4014

C

6

160

Course type (compulsory or optional)

Compulsory

Course level (study cycle)

Bachelor

Semester the course is delivered

Spring

Study form (face-to-face or distant)

Face-to-face

Course title in Lithuanian

MOLEKULINĖ GENETIKA

Course title in English

MOLECULAR GENETICS

Short course annotation in Lithuanian

Molekulinės genetikos principai ir pagrindinės sąvokos. Genų molekulinė struktūra ir funkcijos, ekspresijos ir reguliavimo m molekulinis mechanizmas. Molekuliniai genų ir genomo tyrimų metodai. Tiesioginė ir atvirkštinė genetika. Bakterijų, mielių, augalų, žmogaus molekulinė genetika. Genų terapija. Molekulinės genetikos panaudojimas diagnostikoje. Vėžio genetika

Short course annotation in English

This program encompasses studies the structure and function of genes, inheritence, expression and regulation at a molecular level. Students will be introduced to useful tools and concepts that enable the study of a wide spectrum of molecular genetics; sample collection; genome scanning with subsequent linkage analysis; mutation detection methods; and the use of animal methods. All of the favorite model organisms used in modern genetic analysis are represented in this program, including phage and viruses, bacteria, yeast and fungi, nematodes, Drosophila, algae, plants, and mice. This program is rounded out by an additional strength in human molecular genetics, a research area of increasing importance.

Prerequisites for entering the course

Basics biology, Biochemistry, General genetics.

Course aim

The aim of the course is to introduce principles and concepts of molecular genetics and to provide basic knowledge of molecular genetic methods and its applications.

Content (topics)

 1. Principles and concepts of molecular genetics
 2. Gene structure and genom
 3. Reverse genetics
 4. Forward genetics
 5. Techniques in molecular genetics:  DNA isolation, mRNA isolation, amplification, PGR, separation and detection and expression
 6. Molecular Genetics of Bacteria
 7. Yeast Molecular Genetics
 8. Plant Molecular Genetics
 9. The Human Genome Project
 10. Gene therapy
 11. Cancer gGenetics
 12. Epigenetic

Practical work (contents):

eucaryotic cell and tissue culture; plasmid DNA preparation; DNA amplification and analysis; genetic transofrmation; and analysis techniques.

Distribution of workload for students (contact and independent work hours)

Lectures – 30 hours, laboratory work – 20 hours,  individual work – 58 hours.

Structure of cumulative score and value of its constituent parts

Final assessment sums the assessments of written final examination (50%), written mid-term examination (20%) and assessment of laboratory works (30%).

Recommended reference materials

No.

 

Publication year

Authors of publication and title

Publishing house

Number of copies in

University library

Self-study rooms

Other libraries 

Basic materials

1.

2011

Lewin‘s GENES edit. J.E.Krebs, D.T.Kilpatrick, E.S. Goldstein.

Jones and Bartlett publishers

1

1

 

2.

2007

J. D. Watson, T.A. Baker, S.P. Bell. Molecular Biology of the Gene

Benjamin-Cummings Publishing Company

 

1

1

3.

2010

T Strachan, A.P. Read. Human Molecular Genetics

Garland Publishing

 

1

1

4.

2006

Sasnauskas K. Molekulinė biologija

Garland Publishing

 

1

1

Supplementary materials

1.

2000-2012

Molecular Genetics and Genomics

http://link.springer.com/journal/438

 

2.

2000-2012

Human molecular genetics

http://hmg.oxfordjournals.org/

 

3.

2003

L. Snyder, W. Champness. Molecular Genetics of Bacteria

ASM Press

 

6.

2005

Seiler J.P., Good laboratory practice, 2nd edition

Springer

 

Course programme designed by

Prof.Dr. Algimantas Paulauskas, Department of Biology, Faculty of Natural Sciences

Additional information