General Biochemistry 3 (BBK 4002) - VDU Biochemijos ir biotechnologijų katedra
Klaida
  • Įvyko klaida įkeliant kanalų duomenys

General Biochemistry 3 (BBK 4002)

Course code

Course group

Volume in ECTS credits

Course hours

BBK4002

C

6

160

Course type (compulsory or optional)

Compulsory

Course level (study cycle)

Bachelor

Semester the course is delivered

Autumn

Study form (face-to-face or distant)

Face-to-face

Course title in Lithuanian

BENDROJI BIOCHEMIJA 3

Course title in English

GENERAL BIOCHEMISTRY 3

Short course annotation in Lithuanian

Dalykas suteikia žinių apie nukleorūgštis, baltymus ir šių molekulių sąveiką ląstelėje, užtikrinančią jos tinkamą augimą, dalijimąsi ir vystymąsi. Dalykas apima plačią ir greitai besikeičiančią mokslo sritį, akcentuojami DNR replikacijos, reparacijos, transkripcijos, baltymų sintezės ir genų reguliacijos skirtinguose organizmuose molekuliniai mechanizmai. Studentai supažindinami su metodais ir eksperimentais, skirtais šių mechanizmų tyrimui, pasitelkiant originalią mokslinę literatūrą, detaliau nagrinėjamos tokios greitai besivystančios sritys kaip chromatino struktūra ir funkcijos, RNR polimerazės dinamika ir genų ekspresijos reguliavimas įvairių rūšių RNR molekulėmis.

Short course annotation in English

This course deals with nucleic acids and proteins and how these molecules interact within the cell to promote proper growth, division, and development. It is a large and ever-changing discipline. This course will emphasize the molecular mechanisms of DNA replication, repair, transcription, protein synthesis, and gene regulation in different organisms. The students will learn the techniques and experiments used to discern these mechanisms, often referring to the original scientific literature. It will take an in-depth look at some rapidly evolving fields, including chromatin structure and function, RNA polymerase dynamics, and regulation of gene expression by different types of RNAs.

Prerequisites for entering the course

Chemical analysis 2, General biochemistry 1 and 2

Course aim

The aim of the course is to provide students with the knowledge on molecular mechanisms of DNA replication, repair, transcription, protein synthesis, and gene regulation in different organisms.

Content (topics)

  1. Macromolecules of the cell. Nucleic acids. DNA replication, replication enzymes. DNA recombination and transposition in prokaryotes and eukaryotes. DNA repair. Hydrolysis of nucleic acids.
  2. Transcription and posttranscriptional RNA processing. RNA functions in gene expression.
  3. Chromatin structure, proteins of chromatin, mechanisms of chromatin reorganization, histone code.
  4. Prokaryotic gene transcription;
  5. Eukaryotic genomes. Unique and repetitive sequences. Human genome. Comparison of large genomes. Significance of genome decoding.
  6. Eukaryotic gene transcription. Gene structure. Transcription factors. Complexes of transcription initiation. Elongation.
  7. Translation. Protein folding and chaperones. Inhibitors of protein synthesis. Protein transport through membranes.
  8. Analysis methods for cell macromolecules and protein analysis.

Practical work (contents):

Plasmid amplification, extraction using MiniPrep, evaluation of concentration, quality and purity. Extraction od RNA form eukaryotic cells, evaluation of concentration, quality and purity. In addition practical work is comprised of the discussion of several recent primary research papers. Students will focus on the data presented in the figures and tables, and small groups will be asked to present figures from the paper. In addition, for each paper, students will be asked to write a short (~2-3 page) review describing the main findings of the paper and the implications of the research.

Distribution of workload for students (contact and independent work hours)

Lectures – 45 hours, seminars – 30 hours, individual work – 79 hours, mid-term and final examination – 6 hours

Structure of cumulative score and value of its constituent parts

Final assessment sums the assessments of written final examination (50%), written mid-term examination (30%) and assessment of seminar works (20%).

Recommended reference materials

No.

 

Publication year

Authors of publication and title

Publishing house

Number of copies in

 

University library

Self-study rooms

Other libraries

 

Basic materials

 

1.

2012

Kadziauskas J. Biochemijos pagrindai: bendrasis vadovėlis. 2nd ed.

Vilniaus universitetas

13

2

 

 

2.

2008

Kadziauskas J. Biochemijos pagrindai: bendrasis vadovėlis.

Vilniaus universitetas

67

3

 

 

3.

2009

Campbell MK., Farrell SO. Biochemistry.

Tomson

 

1

 

 

4.

2005

Karp G. Cell and Molecular Biology.

John Wiley&Sons

 

 

1

 

Supplementary materials

1.

2002

Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K.., Walter, P. Molecular Biology of the Cell

Garland Science

 

2.

2000

Lodish, H., Baltimore, D., Berk, A., Zipursky, S.L., Matsudaira, P., Darnell J. Molecular Cell Biology, 4th ed.,

Scientific American Books, N.Y.,.

 

3.

2004

J. D. Watson et all. Molecular biology of the gene (5th. Ed.),

CSHL press

 

Course programme designed by

Doc.dr.R. Žūkienė, Department of Biochemistry and Biotechnologies and prof.dr.S Šatkauskas Department of Biology, Faculty of Natural Sciences

Additional information