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General Biochemistry 2 (BBK 3007)

Course code

Course group

Volume in ECTS credits

Course hours

BBK3007

C

6

160

Course type (compulsory or optional)

Compulsory

Course level (study cycle)

Bachelor

Semester the course is delivered

Spring

Study form (face-to-face or distant)

Face-to-face

Course title in Lithuanian

BENDROJI BIOCHEMIJA 2

Course title in English

GENERAL BIOCHEMISTRY 2

Short course annotation in Lithuanian

Biochemijos II kurso tikslas suteikti žinių apie pagrindinius gyvų sistemų funkcionavimo principus bei medžiagų apykaitos pagrindinius dėsningumus, jų tarpusavio ryšius. Kurso pagrindinės temos: Sacharidų  metabolizmas. Glikolizė, Gliukoneogenezė. Trikarboksirūgščių ciklas. Biocheminių procesų erdvėskyra ir ryšys ląstelėje. Membranos baltymai bei medžiagų pernašos per biologines membranas. Oksidacinis fosforilinimas. Fotosintezė. Lipidų metabolizmas Neutralių riebalų skaidymas. Riebalų rūgščių sintezė. Baltymų apykaita. Azoto fiksacija. Aminorūgščių biosintezė. Nukleorūgščių metabolizmas. Ryšys tarp sacharidų, baltymų ir riebalų apykaitos. Gautos žinios leis suprasti gyvųjų sistemų cheminės sandaros bei funkcionavimą, įgaus praktinio darbo su biomolekulėmis laboratorinius.

Short course annotation in English

The course introduces basic knowledge about organization and principles of metabolism in living system. The course inludes: general aspects of metabolism; metabolism of carbohydrates; principles of membrane transport; pathways for energy transformation in organisms; oxidative phosphorylation; principles of synthesis of main biochemical compounds; integration and regulation of mainstream metabolic pathways. In practicals technical skills for studies in biochemistry are gained. Teaching methods are: lectures and laboratory works.

Prerequisites for entering the course

Inorganic and Bioinorganic chemistry, Organic chemistry, Chemical analysis 2, Biochemistry 1

Course aim

The aim of course is to introduce basic knowledge about organization and principles of metabolism in living system.

Content (topics)

 1. Introduction. Definitions of metabolism, catabolism and anabolism.
 2. Basic thermodynamics of biological systems
 3. Carbohydrates metabolism. Glycolysis.
 4. Citric acid cycle.
 5. Glycogen metabolism. Metabolism of hexoses other than glucose.
 6. Movement through membranes. Transport mechanisms, different types of cellular transport.
 7. Electron transport and oxidative phosphorylation.
 8. Photosynthesis. Calvin cycle
 9. Gluconeogenesis, pentose phosphate pathway.
 10. Lipid metabolism. Fatty oxidation. Ketone bodies.
 11. Biosynthesis of fatty acids.
 12. Protein and amino acids degradation. Urea cycle.
 13. Nucleotide metabolism. Nucleotide degradation.
 14. Biosynthesis of amino acids and nucleotides. Nitrogen fixation.
 15. Overview of protein synthesis

Practical work (contents):

The course includes 10 practical works. 1) Methabolism of carbohydrates. Isolation of glycogen from muscles and liver; 2) Determination of lactic acid in the muscles; 3) Lipid metabolism. Determination of pancreatic phospholipase activity. 4) Effect of bile on lipase activity; 5) Protein metabolism Protein digestion by pepsin; 6) Amino acids transamination;  7) Liver Mitochondria isolation. Determination of mitochondrial protein content; 8) Oxidative phosphorylation. Evaluation of mitochondrial functional parameters; 9) Evaluation mitochondrial function by NAD(P)H fluorescence; 10) Measurement of mitochondrial membrane potential

Distribution of workload for students (contact and independent work hours)

Lectures – 45 hours, laboratory work– 30 hours, individual work – 85 hours.

Structure of cumulative score and value of its constituent parts

Final assessment sums the assessments of written final examination (50%), written mid-term examination (25%) and assessment of laboratory works (25%).

Recommended reference materials

No.

 

Publication year

Authors of publication and title

Publishing house

Number of copies in

University library

Self-study rooms

Other libraries 

Basic materials

1.

2008

Kadziauskas J. Biochemija

VU leidykla

70

1

 

2.

2003

A.Praškevičius, L.Ivanovienė, N.Stasiūnienė, J.Burneckienė, H.Rodovičius, L.Lukoševičius, D.Kondrotas. Biochemija

Printed KMU, Kaunas

22

 

 

3.

2010

Z. Naučienė, R. Žūkienė, D. Vieželienė, V. Mildažienė. Biochemijos laboratoriniai darbai 

Vytauto Didžiojo universitetas

electronic edition

 

 

4.

2000

D. Vieželienė, V. Mildažienė, Z. Naučienė, Biochemijos laboratoriniai darbai

Vytauto Didžiojo universitetas, Kaunas

39

3

 

Supplementary materials

1.

2004

D.L. Nelson, M.M. Cox, A.T. Lehninger. Principles of biochemistry

Worth Publishers, N.Y.

 

2.

2006

Donald Voet, Judith G. Voet. Fundamentals of Biochemistry, 2nd ed.

Printed in USA

 

Course programme designed by

Doc.dr. Zita Naučienė, prof.habil.dr. Rimantas Daugelavičius, Department of Biochemistry and Biotechnologies, Faculty of Natural Sciences,

Additional information